Instructions (Part IV): Place the slide on the microscope stage and secure it with the stage clips. Start with the lowest power objective lens. Use the coarse focus knob to move the stage up, bringing the slide closer to the objective lens. Look through the eyepiece and slowly adjust the focus until the letter “e” comes into view. Once the “e” is roughly in focus, switch to a higher power if desired and use the fine focus knob to clarify the image. Observe how the image of the “e” appears upside down and reversed. This is a normal characteristic of compound microscopes. Experiment with moving the slide up, down, left, and right. What do you notice? Share your thoughts with your partner and/or group. See the video below as a guide: